URL: http://www.addgene.org/47655
Proper Citation: RRID:Addgene_47655
Insert Name: PesaR-P
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23879176
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pCS-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: PesaR-P (274 bp, indicated as lowercase letters in the partial sequence) was clonedwas cloned between XhoI and BamHI in the same was as wt PesaR in pCS-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pCS-PesaRlux for full plasmid backbone sequence and plasmid map.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org