URL: http://www.addgene.org/47327
Proper Citation: RRID:Addgene_47327
Insert Name: Cas9
Organism: Other
Bacterial Resistance: Kanamycin
Defining Citation: PMID:24873830
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET-28b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to express a His-tagged Cas9 protein in E. coli and purify it by nickel-based affinity purification.The Cas9 protein and sgRNA were incubated at room temperature to form the Cas9 protein/sgRNA complex in vitro, which was then microinjected into zebrafish zygotes. See publication for more details.
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Source: Addgene