URL: http://www.addgene.org/46294
Proper Citation: RRID:Addgene_46294
Insert Name: codon optimized Cas9
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24478335
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript-KS(+); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid was created by subcloning the Hsp70-Cas9 containing NotI fragment from Addgene plasmid 45945 in NotI digested pBluescript-KS(+). For more information on FlyCRISPR plasmids please refer to: https://www.addgene.org/browse/article/6834/. Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.
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Source: Addgene