URL: http://www.addgene.org/45809
Proper Citation: RRID:Addgene_45809
Insert Name: a1Ha
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9670923
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: contains nt −74 to 7461 of α1H 5' end cloned into EcoRV site of pcDNA3: GATATCATCGATGAATTC then CGGGCGGTG as reported in Genbank AF051946. 3' ends same place as Genbank entry (CTCCCCTCCG), then a bit of the PUC18 polylinker, before the XbaI site of pcDNA3.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org