URL: http://www.addgene.org/45482
Proper Citation: RRID:Addgene_45482
Insert Name: TRPM7
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11385574
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Other, Tetracycline Inducible; Bacterial Resistance:Ampicillin
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
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Source: Addgene