URL: http://www.addgene.org/44238
Proper Citation: RRID:Addgene_44238
Insert Name: Laconic (LACtate Optical Nano Indicator from CECs)
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23469056
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5418; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression, lactate biosensor; Bacterial Resistance:Ampicillin
Comments: Laconic is a genetically-encoded biosensor that detects lactate in the physiological range. The sensor was based on LldR, a bacterial transcription regulator that consists of a lactate-binding/regulatory domain and a DNA-binding domain. Laconic consists of a N-terminal mTFP, followed by the LldR flanked by linkers, and finally a C-terminal Venus. See Addgene plasmid# 46307: NLS-Laconic/pCMV-myc-nuc for a nuclear-targeted version of Laconic: http://www.addgene.org/46307/ .
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Source: Addgene