URL: http://www.addgene.org/43914
Proper Citation: RRID:Addgene_43914
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:22174877
Vector Backbone Description: Backbone Marker:Addgene Plasmid 19778; Backbone Size:8600; Vector Backbone:FU-tet-o-hOct4; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: To make a drug-inducible lentiviral vector that is compatible with Gateway recombination (Invitrogen), the depositing laboratory removed the OCT4 open reading frame from FU-tetO-hOCT4 (Addgene plasmid 19778) and replaced it with a Gateway cassette cloned from pEF-DEST51 (Invitrogen) to generate FU-tetO-Gateway. ORFs that lack stop codons can be cloned into this plasmid to express proteins with a C-terminal V5 epitope tag. Please note that Addgene's sequencing results identified a single nucleotide insert between bp# 2913/2914 and single nucleotide mismatches at bp# 3303 and 4652 when compared to the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
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Source: Addgene