URL: http://www.addgene.org/40999
Proper Citation: RRID:Addgene_40999
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20733051
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998). The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended). This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines. For subcloning, the following restriction sites are present: Between the CMV promoter and the Gateway cassette: PmeI (non-unique) Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pcDNA5/FRT/TO-Venus-Flag-Gateway (1124).
No alerts have been found for pcDNA5/FRT/TO-Venus-Flag-Gateway (1124).
Source: Addgene