URL: http://www.addgene.org/39238
Proper Citation: RRID:Addgene_39238
Insert Name: 8 copies of the 234bp mouse γ-satellite repeat
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11114330
Vector Backbone Description: Backbone Size:3806; Vector Backbone:low copy pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Comments: The four satellite repeats, which are duplicated in the construct, were generated by PCR from mouse DNA, so they represent a native mouse satellite array. The sequence is a consensus, however deviation from the consensus sequence ranging from 0.9 to 9.1% (mean deviation 3.9%) has been reported. The construct is mainly used as a FISH probe for visualising pericentromeric regions in mouse cells and this is unlikely to be affected by minor sequence variations. Another important point about this construct is that contains 8 direct repeats and the 234 bp satellite sequence also contains internal repeats. This will make it prone to rearrangements when it is grown up for isolation, which means that it will be difficult to completely guarantee its integrity. For reference please see - Vissel, B. and Choo KH. (1989). Mouse major (gamma) satellite DNA is highly conserved and organised into extremely long tandem arrays: Implications for recombination between non-homologous chromosomes. Genomics 5, 407-414.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org