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Plasmid Name
RRID:Addgene_36325 RRID Copied  
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RRID:Addgene_36325
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Plasmid Information

URL: http://www.addgene.org/36325

Proper Citation: RRID:Addgene_36325

Insert Name: D1ER second generation cameleon (calcium sensor) targeted to ER

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:15585581

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and Citrine. D1ER does not bind wild-type calmodulin. It has a Kd1' of 0.8 uM and a Kd2' of 60 uM. D1ER is retained in the ER with a C-terminal KDEL sequence. See also Palmer & Tsien (2006) Nature Protocols.

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Data and Source Information

Source: Addgene