URL: http://www.addgene.org/36158
Proper Citation: RRID:Addgene_36158
Insert Name: Sec23A
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21045114
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech) to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI) and cloned into pLVX-Puro–mRuby.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org