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Plasmid Name
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RRID:Addgene_32426
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Plasmid Information

URL: http://www.addgene.org/32426

Proper Citation: RRID:Addgene_32426

Insert Name: Flag-mCherry-T2A-GFP-T2A-neo

Bacterial Resistance: Ampicillin

Defining Citation: PMID:22355594

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:Ac5/V5-HIS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin

Comments: For multicistronic expression in insect cells. FLAG-mCherry, GFP, or Neo can be removed or replaced. N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible. Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.

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Data and Source Information

Source: Addgene