URL: http://www.addgene.org/31865
Proper Citation: RRID:Addgene_31865
Insert Name: 24xMS2 Stem Loops - Stable
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9809065
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4Blunt-TOPO; Vector Types:; Bacterial Resistance:Ampicillin
Comments: The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination. For mammalian cells they recommend the following plasmids: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561) HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718) For yeast cells they recommend the following plasmids: pET246-pUC57 12xMS2V6 (Plasmid #104390) pET259-pUC57 24xMS2V6 (Plasmid #104391) pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392) pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393) This version of the 24xMS2 stem loops has been engineered to allow stable propagation in bacteria. Loss of repeats is still possible, but occurs significantly less often than with previous version. This plasmid is considered the replacement for pSL-MS2-24X (Addgene plasmid# 27120). The 24xMS2 stem loop cassettes in this plasmid were generated from two non-identical stem-loops to reduce unnecessary redundancy and the chance of recombination in bacteria. The sequence for a single cassette (with the two non-identical stem-loops in uppercase) is: tacggtacttattgccaagaaaGCACGAGCATCAGCCGTGC ctccaggtcgaatcttcaaaCGACGACGATCACGCGTCGctccagtattccagggttcatc
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Source: Addgene