URL: http://www.addgene.org/29435
Proper Citation: RRID:Addgene_29435
Insert Name: c-Myc shRNA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19745813
Vector Backbone Description: Backbone Marker:David Baltimore (Caltech) and Valerie; Backbone Size:7000; Vector Backbone:FUGW, modified; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: sh1368 sequence= GACGAGAACAGTTGAAACA The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008). To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation. See the attached document for a map from Lien et al., 2008 for vector information.
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Source: Addgene