URL: http://www.addgene.org/29435
Proper Citation: RRID:Addgene_29435
Insert Name: c-Myc shRNA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19745813
Vector Backbone Description: Backbone Marker:David Baltimore (Caltech) and Valerie; Backbone Size:7000; Vector Backbone:FUGW, modified; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: sh1368 sequence= GACGAGAACAGTTGAAACA The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008). To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site–neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation. See the attached document for a map from Lien et al., 2008 for vector information.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org