URL: http://www.addgene.org/27118
Proper Citation: RRID:Addgene_27118
Insert Name: MS2 binding sites (6)
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9809065
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:3422; Vector Backbone:pSL1180; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination. For mammalian cells they recommend the following plasmids: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561) HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718) For yeast cells they recommend the following plasmids: pET246-pUC57 12xMS2V6 (Plasmid #104390) pET259-pUC57 24xMS2V6 (Plasmid #104391) pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392) pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393) Loop sequence: acatgaggatcacccatgt Addgene's sequencing result contains 1 nucleotide mismatch at bp# 345 when compared to the full plasmid sequence provided by the depositing laboratory.
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Source: Addgene