URL: http://www.addgene.org/26741
Proper Citation: RRID:Addgene_26741
Insert Name: ensconsin
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19060892
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin
Comments: The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal of Cell Science 112, 4243-4255. Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org