URL: http://www.addgene.org/26741
Proper Citation: RRID:Addgene_26741
Insert Name: ensconsin
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19060892
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin
Comments: The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal of Cell Science 112, 4243-4255. Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.
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Source: Addgene