URL: http://www.addgene.org/26647
Proper Citation: RRID:Addgene_26647
Insert Name: Cre
Organism: Bacteriophage P1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cre was excised from pcDNA3Cre as a directionaly sticy blunt by first cutting with HindIII, then blunting with Klenow, and finally cutting with NotI. The directional Cre fragment was cloned into the pCAG backbone. The eGFP cassette was removed from the pCAG-GPF backbone using SmaI (which left a blunt end) and NotI. Cre was then subcloned directionally into the pCAG backbone. This construct does NOT express GFP.
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Source: Addgene