URL: http://www.addgene.org/26529
Proper Citation: RRID:Addgene_26529
Insert Name: miR-155
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18299402
Vector Backbone Description: Backbone Marker:Addgene plasmid 12282; Backbone Size:6500; Vector Backbone:pMIG-w; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.
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Source: Addgene