URL: http://www.addgene.org/252243
Proper Citation: RRID:Addgene_252243
Bacterial Resistance: Ampicillin
Defining Citation: PMID:40631108
Vector Backbone Description: Backbone Marker:Jay Shendure, JB Lalanne, Sam Regalado; Vector Backbone:p051_PB_tornado; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A FISSEQ capable barcode must first be cloned into this plasmid via BsmBI/Esp3I and a gene or ORF library must be cloned in via SapI before transfection. This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome. Please visit https://www.biorxiv.org/content/10.1101/2025.07.03.663081v1 for bioRxiv preprint.
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No rating or validation information has been found for PB-circBC-UCOE-EF1a-mEGFP(NTag)-IRES-PuroR-WPRE.
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Source: Addgene