URL: http://www.addgene.org/25040
Proper Citation: RRID:Addgene_25040
Insert Name: miR-9 sponge
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20173740
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: 8 tandem binding sites to human miR-9. Repeated sequence is TCATACAGCTATATACCAAAGA. Annealed oligonucleotides for tandem miR-9-binding sites were ligated into the pcDNA5-CMV-d2eGFP vector (Invitrogen) digested with XhoI and ApaI. The gfp mRNA, along with the miR-9 sponge sequence in the 3′ UTR was then subcloned into the pBabe-puro vector digested with BamHI and SalI. Please note that the total insert size between BamHI and SalI is approximately 1327bp, as it includes d2EGFP, the miR-9 binding sites and a BGH polyA terminator.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org