URL: http://www.addgene.org/248333
Proper Citation: RRID:Addgene_248333
Insert Name: gRNAscaffold-tRNA
Organism: P. patens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Golden Gate fragments are amplified by PCR, with primers designed to produce half of each gRNA followed by BsaI recognition sites. Complete gRNA sequences to be reconstituted upon BsaI digestion and ligation during the Golgen Gate reaction.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org