URL: http://www.addgene.org/24526
Proper Citation: RRID:Addgene_24526
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18323534
Vector Backbone Description: Backbone Marker:Richard Mulligan; Backbone Size:0; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: The pHAGE vector was modified for dual transgenesis as follows: cDNA encoding a variant of the red fluorescent protein adapted from Discosoma sp. (DsRed-Express; Clontech, Mountain View, CA) was amplified by PCR attaching NotI and BamH1 restriction sites to 5' and 3' ends, respectively. This amplicon was cloned into the pHAGE backbone in the first gene expression position by ligation to NotI/BamH1 cohesive ends. Next, enhanced green fluorescence protein (GFP; Clontech) cDNA was generated by PCR attaching NdeI and ClaI sites to the 5' and 3' ends, respectively, for ligation into the second gene position of pHAGE. Immediately upstream of the dsRed or GFP ATG start site, the indicated promoter fragment (cytomegalovirus [CMV], 584 bp; ubiquitin C [UBC], 397 bp]) was inserted by standard cloning techniques. See map for additional details.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org