URL: http://www.addgene.org/21836
Proper Citation: RRID:Addgene_21836
Insert Name: EGFP
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:4569; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The Puro resistance gene of pBABE puro was removed by Hind3-Cla1 digestion and replaced by the CDS of a EGFP that has a farnysylation signal in its C-term. The membrane association of EGFP is instrumental in detecting the protein in FACScan procedures that would otherwise lead to loss of GFP fluorescence.
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Source: Addgene