URL: http://www.addgene.org/21374
Proper Citation: RRID:Addgene_21374
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The HIV-dTomato plasmid encodes a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with dTomato. This construct was made in Zena Werb's laboratory and has not been published. Note: This plasmid contains dTomato and not the tandem conjugate TdTomato.
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Source: Addgene