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Plasmid Name
RRID:Addgene_21164 RRID Copied  
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RRID:Addgene_21164
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Plasmid Information

URL: http://www.addgene.org/21164

Proper Citation: RRID:Addgene_21164

Insert Name: Klf4, cMyc

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:19325077

Vector Backbone Description: Backbone Size:5500; Vector Backbone:pSIN4-EF2-IRES-Pur; Vector Types:Mammalian Expression, Lentiviral, Other, reprogramming; Bacterial Resistance:Ampicillin

Comments: After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. Infections with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-EF2-N2L have a significantly greater reprogramming efficiency than our original 4 lentiviruses. NOTE: Addgene's quality control sequence found a K269T in Klf4. This mutation is not known to affect function.

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Data and Source Information

Source: Addgene