URL: http://www.addgene.org/21076
Proper Citation: RRID:Addgene_21076
Insert Name: Atg4B(C74A)
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:18768752
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmStrawberry C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Atg4B insert also contains V190A and T325K compared to listed reference sequence.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org