URL: http://www.addgene.org/20972
Proper Citation: RRID:Addgene_20972
Insert Name: Histone cluster 1, H2bb
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19896442
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The mCherry cDNA was obtained from Dr. Roger Tsien.
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Source: Addgene