URL: http://www.addgene.org/208045
Proper Citation: RRID:Addgene_208045
Insert Name: Ub
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:37996419
Vector Backbone Description: Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity.
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Source: Addgene