URL: http://www.addgene.org/208034
Proper Citation: RRID:Addgene_208034
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22355594
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:Ac5/V5-HIS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: For multicistronic expression in insect cells. FLAG-mCherry, GFP, or Blast can be removed or replaced (swapped Nhe1-Xho1 NeoR from Addgene ID: 32426 for BlastR). N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible. Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org