URL: http://www.addgene.org/20061
Proper Citation: RRID:Addgene_20061
Insert Name: MSP1D1
Organism: synthetic gene
Bacterial Resistance: Kanamycin
Defining Citation: PMID:15025475
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: NOTE: This plasmid is supplied in DH5alpha. For expression, it must be prepped and retransformed into BL-21(DE3); high oxygenation level This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
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Source: Addgene