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Plasmid Name
RRID:Addgene_194469 RRID Copied  
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RRID:Addgene_194469
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Plasmid Information

URL: http://www.addgene.org/194469

Proper Citation: RRID:Addgene_194469

Insert Name: BtGH84

Organism: B. thetaiotaomicron VPI-5482

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Size:3159; Vector Backbone:pRN3P-H3.3-K9R-Gfp; Vector Types:Bacterial Expression, for In Vitro Transcription from T3 promoter after linearization; Bacterial Resistance:Ampicillin

Comments: Paper describing the insert: Dennis, R. J. et al. Structure and mechanism of a bacterial β-glucosaminidase having O-GlcNAcase activity. Nat. Struct. Mol. Biol. 13, 365–371 (2006). Paper of lab donating backbone, also using pRN3P backbone: Eid, A., Rodriguez-Terrones, D., Burton, A. & Torres-Padilla, M. E. SUV4-20 activity in the preimplantation mouse embryo controls timely replication. Genes Dev. 30, 2513–2526 (2016). Paper using plasmid from which Btgh insert was amplified: Boulard, M., Edwards, J. R. & Bestor, T. H. Protein O-GlcNAcylation Silences Methylated Promoters. Please visit https://www.biorxiv.org/content/10.1101/2024.01.22.576677v1 for bioRxiv preprint.

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Data and Source Information

Source: Addgene