URL: http://www.addgene.org/179904
Proper Citation: RRID:Addgene_179904
Insert Name: RAB11B, member RAS oncogene family
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35708998
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Comments: Here donor for DExogron Rab11b knock in. DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation. Lentiviral delivery of AtAFBP2 and TetOn 3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org