URL: http://www.addgene.org/17870
Proper Citation: RRID:Addgene_17870
Insert Name: 3x NFAT binding sequence
Bacterial Resistance: Ampicillin
Defining Citation: PMID:1377362
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51. The plasmid can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue including lymphocytes, neurons, osteoblasts, skin, endothelium, skeletal and heart muscle (Shaw et al Science 1988; Crabtree and Schreiber Cell 138,210, 2009). Based on Addgene's full plasmid sequence, the NFAT binding sequence appears to be ACGCCTTCTGTATGAAACAGTTTTTCCTCC.
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Source: Addgene