URL: http://www.addgene.org/17870
Proper Citation: RRID:Addgene_17870
Insert Name: 3x NFAT binding sequence
Bacterial Resistance: Ampicillin
Defining Citation: PMID:1377362
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51. The plasmid can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue including lymphocytes, neurons, osteoblasts, skin, endothelium, skeletal and heart muscle (Shaw et al Science 1988; Crabtree and Schreiber Cell 138,210, 2009). Based on Addgene's full plasmid sequence, the NFAT binding sequence appears to be ACGCCTTCTGTATGAAACAGTTTTTCCTCC.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org