URL: http://www.addgene.org/177839
Proper Citation: RRID:Addgene_177839
Bacterial Resistance: Kanamycin
Defining Citation: PMID:35995792
Vector Backbone Description: Backbone Size:3282; Vector Backbone:pK18sB; Vector Types:Bacterial gene replacement; Bacterial Resistance:Kanamycin
Comments: The sequence for this plasmid has been deposited with Genbank (Accession # OK423783). Sequences were derived from pK18mobsacB (ATCC 87097), which was originally described in Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69–73. doi:10.1016/0378-1119(94)90324-7
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org