URL: http://www.addgene.org/177316
Proper Citation: RRID:Addgene_177316
Insert Name: mGAD65(delE1) promoter, GABAergic interneurons specific promoter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33588899
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please note in Addgene's NGS result the M13 ori is inverted and there are few ambiguous bases in one of the ITRs. ITRs can be difficult to sequence. Additionally an 8bp deletion of a GT repeat was identified in the mGAD65(delE1) promoter compared to the depositor's reference sequence. The depositor confirmed that the plasmid is still functional.
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No rating or validation information has been found for pAAV mGAD65(delE1) GFP WPRE SV40pA.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org