URL: http://www.addgene.org/174448
Proper Citation: RRID:Addgene_174448
Insert Name: RNase H1
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:34232287
Vector Backbone Description: Backbone Marker:California Institute for Quantitative Biosciences, University of California, Berkeley MacroLab; Backbone Size:6075; Vector Backbone:1GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Expression of His-GFP-RNase H1 D210N was induced with 0.3 mM IPTG at 16C for 18-20 hours in BL21(DE3)/Rosetta after OD600 reaching 0.3-0.5.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org