URL: http://www.addgene.org/164896
Proper Citation: RRID:Addgene_164896
Insert Name: Minimal human genome-wide CRISPR-Cas9 gRNA library
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33478580
Vector Backbone Description: Backbone Size:9314; Vector Backbone:pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W (Addgene Plasmid 153033); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: For the plasmid library, total read counts per sgRNA were calculated using the count_spacers python script: Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or with cell lines already expressing Cas9
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org