URL: http://www.addgene.org/164896
Proper Citation: RRID:Addgene_164896
Insert Name: Minimal human genome-wide CRISPR-Cas9 gRNA library
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33478580
Vector Backbone Description: Backbone Size:9314; Vector Backbone:pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W (Addgene Plasmid 153033); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: For the plasmid library, total read counts per sgRNA were calculated using the count_spacers python script: Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or with cell lines already expressing Cas9
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Source: Addgene