URL: http://www.addgene.org/16399
Proper Citation: RRID:Addgene_16399
Insert Name: AdEasier-1 bacterial cells
Organism: E. coli
Bacterial Resistance: Ampicillin and Streptomycin
Defining Citation: PMID:9482916
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin and Streptomycin
Comments: AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system. AdEasier is a trademark of the Johns Hopkins University.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org