URL: http://www.addgene.org/15912
Proper Citation: RRID:Addgene_15912
Insert Name: CAG-STOP-eGFP-ROSA26TV
Organism: DNA derived from yeast, E. coli transposon Tn5 Corynebacterium aequoria (jellyfish)
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17463289
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSII KS; Vector Types:Cre/Lox, /STOP; Bacterial Resistance:Ampicillin
Comments: See author's map and attached PDF for more information. The plasmid is also used in: Xiao C, Calado DP, Galler G, Thai TH, Patterson HC, Wang J, Rajewsky N, Bender TP, Rajewsky K. MiR-150 Controls B Cell Differentiation by Targeting the Transcription Factor c-Myb. Cell. 2007 Oct 5;131(1):146-59. Note that there are some differences between the author's theoretical sequence and Addgene's quality control sequence. The differences do not appear to be in critical regions required for plasmid function.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org