URL: http://www.addgene.org/155184
Proper Citation: RRID:Addgene_155184
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35567999
Vector Backbone Description: Backbone Size:9512; Vector Backbone:Addgene #155182; Vector Types:Mammalian Expression, Other, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Comments: The T2A-Puro sequence was re-amplified and cloned into the backbone to remove BbsI restriction site in the T2A sequence. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns and Ms are the gRNA sequence and its reverse-complement counterpart: FWD:5'- AAACNNNNNNNNNNNNN RVS: 5'-AAAAMMMMMMMMMMM
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No rating or validation information has been found for XLone-Puro Cas13d-eGFP U6 BbsI.
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Source: Addgene