URL: http://www.addgene.org/14757
Proper Citation: RRID:Addgene_14757
Insert Name: membrane-bound EGFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17209010
Vector Backbone Description: Backbone Size:6122; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus. The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments. Addgene's NGS finds an IS4-like element (ISVsa5 family transposase) in the backbone that does not affect the overall plasmid function.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org