URL: http://www.addgene.org/14585
Proper Citation: RRID:Addgene_14585
Insert Name: Runx1
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16314494
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The Runx1 insert in this plasmid has an extra 34 amino acids appended onto the c-terminus. The depositor states that this does not affect plasmid function and the plasmid should behave according to the associated publication. Using PCR cloning, full-length Runx1 (1-451) was cloned into the HindIII/XhoI sites of pcDNA3.1Hygro. 5' primer has Kozak and Flag epitope-tag sequences. The plasmid was used in western blot and coimmunoprecipitation.
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No rating or validation information has been found for pcDNA3.1 Flag Runx1 FL (P#1790).
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Source: Addgene