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Plasmid Name
RRID:Addgene_14129 RRID Copied  
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RRID:Addgene_14129
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Plasmid Information

URL: http://www.addgene.org/14129

Proper Citation: RRID:Addgene_14129

Insert Name: TSC2

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12150915

Vector Backbone Description: Backbone Marker:FLAG (MDYKDDDDK) was cloned between HindIII and BamHI of pcDNA3; Backbone Size:5500; Vector Backbone:pcDNA3-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: The first 276 base pairs of TSC2 were PCR-amplified from pcDNA6-TSC2 and inserted between the BamHI and NotI sites in frame with the FLAG-coding sequence in pcDNA3-FLAG. The remainder of TSC2 was subcloned from pcDNA6-TSC2 and inserted between the NotI and XbaI sites of the above construct. Diagnostic digests reveal an extra BamHI site in this plasmid. BamHI will not linearize the construct.

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Data and Source Information

Source: Addgene