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pBabe puro Myr HA PIK3CA

RRID:Addgene_12523

Proper Citation

RRID:Addgene_12523

Insert Name

PIK3CA Myr phosphoinositide 3-kinase, PI3K, p110 alpha CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha

Relevant Mutations

Myristoylation. Constitutively active allele; I143V

Organism

H. sapiens (human)

Notes

To make an amino-terminal myristoylated allele of p110, a coding sequence for the myristoylation domain of c-Src was fused in-frame with a HA-tagged PIK3CA.

Reference

PMID:16339315

Vector Backbone

Vector backbone:pBabe puro; Bacteria resistance:; Selectable marker: Vector type:Mammalian Expression, Retroviral; Backbone marker:Available at Addgene (plasmid #1764); Backbone size:5150