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Plasmid Name
RRID:Addgene_12456 RRID Copied  
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RRID:Addgene_12456
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Plasmid Information

URL: http://www.addgene.org/12456

Proper Citation: RRID:Addgene_12456

Insert Name: TCF/LEF binding sites

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12699626

Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4871; Vector Backbone:pTA-Luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin

Comments: This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site). Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.

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Data and Source Information

Source: Addgene