URL: http://www.addgene.org/122563
Proper Citation: RRID:Addgene_122563
Insert Name: Gbait-hsp70-QF2-SV40pA
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34878403
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:Other, Cloning; Bacterial Resistance:Ampicillin
Comments: This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545. The QF2 sequence used was described in Riabinina et al. “Improved and expanded Q-system reagents for genetic manipulations” Nature methods vol. 12,3 (2015): 219-22, 5 p following 222.
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Source: Addgene