URL: http://www.addgene.org/12250
Proper Citation: RRID:Addgene_12250
Insert Name: TetR-KRAB fusion and dsRED
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12885912
Vector Backbone Description: Backbone Size:11985; Vector Backbone:pLV-tTRKRAB; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Older, double-copy vector system for conditional expression of siRNA. Has been replaced by the all-in-one vector system from Trono/Aebischer lab (e.g. see Addgene plasmid 11643). For conditional expression of siRNA, the target cells need to be cotransduced with both pLVTH/pLVTHM and pLV-tTRKRAB/pLV-tTRKRAB-Red to achieve conditional expression of siRNA. Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pLV-tTRKRAB-red.
No alerts have been found for pLV-tTRKRAB-red.
Source: Addgene