URL: http://www.addgene.org/115163
Proper Citation: RRID:Addgene_115163
Insert Name: Sth1 sgRNA scaffold
Organism: Streptcoccus thermophilus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28165460
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Comments: The group constructed this plasmid in a BSL1 laboratory for the purpose of expressing them in BSL2 organism (such as M. smegmatis) or BSL3 (such as M. tuberculosis). Otherwise, if it's only in E. coli, then it is BSL1.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org