URL: http://www.addgene.org/11313
Proper Citation: RRID:Addgene_11313
Insert Name: 4 bulged binding sites for CXCR4 siRNA antisense
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15014042
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
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Source: Addgene