URL: http://www.addgene.org/111375
Proper Citation: RRID:Addgene_111375
Insert Name: TERRA polyA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27537914
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti4/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please see the Addgene Partial NGS Result for the verified backbone. Please note this result contains only a part (around 670 bps) of the 800 bps telomere sequence repeats, with further variant repeats not seen by Sanger sequencing by the Depositor. This plasmid may be prone to recombination due to the telomere hexanucleotide repeats, and therefore screening multiple colonies for the correct insert length is recommended before proceeding with downstream work
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org